New Trial Results Suggest High Concentrate-Diets Can Cause Endotoxin-Induced Liver Issues in Dairy Cows

The poster, entitled Opens external link in new windowInfluence of a high concentrate diet on rumen pH, fecal endotoxin concentration, and liver enzymes in lactating dairy cows won first prize at the 55th Consultation Meeting of the Bavarian Animal Nutrition Society (Bayerische Arbeitsgemeinschaft Tierernährung or BAT) on 12 October 2017 in Freising, Germany.

Effects of high concentrate diets

The energy and nutrient requirement of dairy cows increases enormously during early lactation. To fulfill these requirements, animal producers often feed concentrate-rich diets to their animals.

However, recent findings suggest that this practice can negatively affect the health status of dairy cows, producing a decrease in the rumen and intestinal pH that  inevitably causes an imbalance of the ruminal and intestinal microbiota, followed by the release of high amounts of endotoxins.

While the liver plays a major role in detoxifying endotoxins, there is a lack of knowledge about the role of liver enzymes in the blood and endotoxin detoxification during feeding concentrate-rich diets.

The aim of the study was indeed to measure the influence of a high concentrate diet on the rumen pH, fecal endotoxin concentration, and liver enzymes in the blood in lactating dairy cows.

The trial

Sixteen lactating Simental cows received a total mixed ratio (TMR) with 40% concentration (dry matter) and 60% roughage (hay:grass silage 20:80 DM). After one week, concentrate amount of the TMR was increased to 60% (dry matter) to induce a subacute acidosis.

During the following four weeks, the pH was monitored as well as the endotoxin concentration in the feces and the liver enzyme activity in the blood of all cows.


The result of this study showed that increasing concentrate amounts can have detrimental health effects on dairy cows, including:

  • a decrease of the average rumen pH to 6.2
  • up to a five-fold increase of fecal endotoxin concentration
  • an increase of liver enzymes in week 4, specifically aminotransferase (AST), glutamatdehydrogenase (GLDH), and gamma-glutamyl transferase (GTT)  

While a healthy cow with a normally functioning liver can cope with a normal load of endotoxins, a large increase in endotoxins, e.g. due to a decrease of the pH from a concentrate-rich diet, may impair liver health and induce an inflammatory response, resulting in performance losses and health issues.

Takeaway message

In practical terms, the trial results suggest that dairy producers should monitor the amount of concentrates in diets, and should also consider feed additives that offer protection against endotoxins, provided that the mode of action has been extensively tested in vivo.

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For further information contact the contributor:

Nicole Reisinger, Dr.nat.techn.

Nicole Reisinger, Dr.nat.techn.
Scientist - Ruminants

BIOMIN Research Center
Technopark 1
3430 Tulln, Austria

Opens window for sending emailnicole.reisinger(at)